Donald Seto, Ph.D.

Microbial Genomics and Diversity
(703) 993-8403, phone

Research Group News and Updates:

Areas of Scientific Interest and Specialization:
     Genomics: DNA sequencing and analysis of viral genomes; phylogeny and mechanisms of adenovirus evolution. 
   Bioinformatics: Development of software tools for genome annotation; tools for whole genome comparisons/analysis. 
   Biodetection: Development and validation of DNA-based molecular diagnostics/microarray pathogen detection (USAF, SG/R: EOS Program) (-ended Oct.31, 2004). 
Current Research Interests

I have extensive experience and interests in bioinformatics, genome analysis, genomics and biochemistry.  An additional expertise is in nucleic acids core facilities development, operations and management from my diverse opportunities.
  •       Academic labs (my laboratory at GMU and Lee Hood laboratory at Caltech)
  •       Government labs (Molecular Endocrinology Branch at NIDDK/NIH and at USAF/EOS)
  •       Commercial labs (DuPont-Merck Pharm., Digital Genes Tech. and GeneSys Tech.)
As a Scientific Advisor to the Surgeon General and the Director of Molecular Diagnostics for the USAF-EOS program, I have project management experience, dealing with research groups at the USAF Surgeon General’s Office, AFIP, NHRC, NRL and LAFB (see below for an acronym key), as well as arranging, directing and managing contract work with Commonwealth Biotechnologies, Inc. and Virapur, Inc.  My lab’s current focus is in software tools development for whole genome data mining and analyses, and in the genomic and bioinformatics analysis of adenovirus genomes, with emphasis on the evolution and natural variation of these genomes.

Software development for whole genome comparisons and analyses
Using virus genome data, e.g., adenoviruses and poxviruses, the Seto Research Group has developed, validated and implemented two net-accessible interactive and “on-the-fly” genome comparison software tools (  The first, GeneOrder, compares two genomes to determine synteny and gene order by using a BLAST algorithm.  The second, CoreGenes, analyzes up to five genomes in order to catalogue genes that have sequence relationships with each other- e.g., ‘alphabet’ of essential genes within a family.  One application of CoreGenes is that it can rapidly predict the function for newly sequenced predicted or hypothetical genes based on other members of the family.  Another application is the presentation of unique and common genes, which may be realized as targets for pharmaceutical drug discovery and for molecular diagnostics.  Recently these tools have been redeveloped for analyzing small bacterial genomes, up to 2Mb. 

Genomics and bioinformatics of human adenoviruses
Several members of the adenoviruses family are important human pathogens.  They are agents in acute respiratory disease (ARD).  While ARD has a low mortality rate, especially in the military training population, the morbidity rate is high.  The Seto Research Group and collaborators have sequenced genomes from sixteen relevant serotypes (1, 4, 6, 7, 14, 16, 21, 34 and 50), and have analyzed and annotated these genomes thoroughly.  An algorithm for annotation has been designed and optimized.  Bioinformatics analysis shows that serotype 4 arose from a zoonotic event, transmission from a chimpanzee host.  Three branches of evolution are suggested, with one branch comprising the majority of the serotypes and species; one branch comprising the species B serotypes; and one ‘relatively recent’ branch comprising the sole member of species E.  Species B is subdivided into B1 and B2, with data suggesting B1 is derived from a zoonotic event and B2 diverging from the original human adenovirus branch and converging with the B1 branch.  Current epidemic outbreak strains of adenoviruses are being studied to determine epidemiology and evolution of pathogenic strains.

Viral and molecular evolution; Natural variation
Current field strains of HAdV-4 isolated from epidemics appear to contain two genome recombination events allowing this serotype to become now the single agent of adenovirus-caused ARD in the military trainee population (at 99%, NHRC Spring 05).  Bioinformatics analyses suggest these recombinations of critical regulatory sites drove the adaptive evolution of this pathogen from a chimpanzee host to a human host.  (re-emerging in the absence of vaccines).

Genomic, bioinformatics and molecular analyses of adenoviruses associated with previously vaccinated individuals
During the course of studies, it was noted that archived strains from the US Navy Health Research Center (Global Emerging Infectious Disease Surveillance- GEIS), collected from individuals who were diagnosed with ARD despite vaccinations (‘Breakthrough’ or ‘coinfection’ strains), contained multiple serotypes of adenoviruses.  One genome was sequenced and analyzed.  This, along with other ARD-relevant adenovirus genomes analyzed earlier, shows that molecular evolution of viral pathogens may be more rapid than earlier thought, and that vaccination with unattenuated viruses may drive recombinations and accelerate the evolution to more problematic substrains.  These genomic studies complement molecular and epidemiologic studies with the collection of adenovirus field strains collected by NHRC.  Molecular recombination and co-infections may be major forces shaping virus adaptive evolution, particularly of pathogenic strains.

Development of advanced molecular diagnostics platforms for epidemic outbreaks surveillance (May 2002 to November 2004)
As part of a Consortium (Epidemic Outbreak Surveillance- EOS) formed by the US Air Force Surgeon General’s Office to develop molecular diagnostics tools for the identification and surveillance of ARD pathogens in the military, the Seto Research Group has provided unique pathogen genome signatures for populating microarray chips.  These set of probes include signatures from several viral and bacterial agents that are identified as pathogens in ARD.  Currently, three versions of the Affymetrix-based Respiratory Pathogen Microarray (RPM) have been designed, and are being tested, validated and implemented in a real-world test bed at LAFB.  This Consortium includes collaborating groups from the Armed Forces Institute of Pathology at Walter Reed Army Medical Center (AFIP), Naval Research Laboratory (NRL), and Wilford Hall/Lackland USAF base (LAFB).  The goal has been to support a rapid response to current epidemic challenges, so methodology has been optimized for sequencing and analyzing viral genomes rapidly, translating this into pathogen signatures for rapid turnaround and for microarray inclusion.  Current epidemic field strains of adenoviruses have been sequenced and analyzed rapidly:  HAdV-4FS (US Air Force), HAdV-4FS (US Army), HAdV-7FS (US Navy), HAdV-3FS (US Navy) and HAdV-5FS (US Army).



Zhang, Q., Ou, J., Lan, W., Wu, X., Zhao, T., Duan, B., Yang, P., Ren, Y., Quan, L., Zhao. W., Seto, D., Chodosh, J., Luo, Z., and Wu, J. (2022). Tracking SARS-CoV-2 Omicron diverse spike gene mutations identifies multiple inter-variant recombination events. Signal Transduction and Targeted Therapy. Apr 2022, in press.

Ji, T., Li, L., Li, W., Zheng, X., Ye, X., Chen, H., Zhou, Q., Jia, H., Chen, B., Lin, Z., Chen, H., Huang, S., Seto, D., Chen, L., and L. Feng. (2021). Emergence and characterization of a putative novel human adenovirus recombinant HAdV-C104 causing pneumonia in Southern China. Virus Evol. 7:veab018.

Ou, J., Zhou, Z., Dai, R., Zhang, J., Zhao, S., Wu, X., Lan, W., Ren, Y., Cui, L., Lan, W., Lu, L., Seto, D., Chodosh, J., Wu, J., Zhang, G., and Zhang, Q. (2021). V367F mutation in SARS-CoV-2 spike RBD emerging during the early transmission phase enhances viral infectivity through increased human ACE2 receptor binding affinity. J. Virol. 95:e0061721.

Kang, J., Ismail, A.M., Dehghan, S., Rajaiya, J. , Allard, M.W., Lim, H.C., Dyer, D.W., Chodosh, J., and Seto, D. (2020). Genomics-based re-examination of the taxonomy and phylogeny of human and simian Mastadenoviruses: an evolving whole genomes approach, revealing putative zoonosis, anthroponosis, and amphizoonosis. Cladistics 36:358-373.

Yan, Y., Ou, J., Zhao, S., Ma, K., Lan, W., Guan, W., Wu, X., Zhang, J., Zhang, B., Zhao, W., Wan, C., Shi, W., Wu, J. Seto, D., and Zhang, Q. (2020). Characterization of Influenza A and B viruses circulating in Southern China during the 2017-2018 season. Front Microbiol. 11:1079.

Yan, Y., Jing, S., Feng, L., Zhang, J., Zeng, Z., Li, M., Zhao, S., Ou, J., Lan, W., Guan, W., Wu, X., Wu, J., Seto, D., and Zhang, Q. (2020). Construction and characterization of a novel recombinant attenuated and replication-deficient candidate human adenovirus type 3 vaccine: "Adenovirus vaccine within an adenovirus vector". Virol Sin. 26:1-11.

Coleman KK, Wong CC, Jayakumar J, Nguyen TT, Wong AWL, Yadana S, Thoon KC, Chan KP, Low JG, Kalimuddin S, Dehghan S, Kang J, Shamsaddini A, Seto D, Su YCF, and GC Gray. (2019). Adenoviral Infections in Singapore: Should New Antiviral Therapies and Vaccines Be Adopted? J Infect Dis. 2019 Sep 30. pii: jiz489. doi: 10.1093/infdis/jiz489. [Epub ahead of print]

Ismail, A.M., Zhou, X., Dyer, D.W., Seto, D., Rajaiya, J., and Chodosh. J. (2019). Genomic foundations of evolution and ocular pathogenesis in human adenovirus species D. FEBS Lett. 593:3583-3608.

Borkenhagen, L.K., Fieldhouse, J.K., Seto D., and Gray, G.C. (2019). Are adenoviruses zoonotic? A systematic review of the evidence. Emerg Microbes Infect 8:1679-1687.

Dehghan S, Seto J, Liu EB, Ismail AM, Madupu R, Heim A, Jones MS, Dyer DW, Chodosh J, and D. Seto. (2019). A Zoonotic Adenoviral Human Pathogen Emerged through Genomic Recombination among Human and Nonhuman Simian Hosts. J Virol. 93. pii: e00564-19.

Zhang J, Kang J, Dehghan S, Sridhar S, Lau SKP, Ou J, Woo PCY, Zhang Q, and D. Seto. (2019). A Survey of Recent Adenoviral Respiratory Pathogens in Hong Kong Reveals Emergent and Recombinant Human Adenovirus Type 4 (HAdV-E4) Circulating in Civilian Populations. Viruses. 11(2) E129.

Singh G, Ismail AM, Lee JY, Ramke M, Lee JS, Dyer DW, Seto D, Rajaiya J, and and J. Chodosh. (2019). Divergent Evolution of E1A CR3 in Human Adenovirus Species D. Viruses 11(2) E143.

Ismail AM, Lee JS, Lee JY, Singh G, Dyer DW, Seto D, Chodosh J, Rajaiya J. (2018). Adenoviromics: Mining the Human Adenovirus Species D Genome. Front Microbiol. 9:2178.

Lee JY, Lee JS, Materne EC, Rajala R, Ismail AM, Seto D, Dyer DW, Rajaiya J, Chodosh J. (2018). Bacterial RecA Protein Promotes Adenoviral Recombination during In Vitro Infection. mSphere 3(3).

Ismail AM, Cui T, Dommaraju K, Singh G, Dehghan S, Seto J, Shrivastava S, Fedorova NB, Gupta N, Stockwell TB, Madupu R, Heim A, Kajon AE, Romanowski EG, Kowalski RP, Malathi J, Therese KL, Madhavan HN, Zhang Q, Ferreyra LJ, Jones MS, Rajaiya J, Dyer DW, Chodosh J, Seto D. (2018). Genomic analysis of a large set of currently-and historically-important human adenovirus pathogens. Emerging Microbes & Infections. 7:10.

Zhang Q, Jing S, Cheng Z, Yu Z, Dehghan S, Shamsaddini A, Yan Y, Li M, Seto D. (2017). Comparative genomic analysis of two emergent human adenovirus type 14 respiratory pathogen isolates in China reveals similar yet divergent genomes. Emerging Microbes & Infections. 6:e92.

Ramke, M., Lee, J.Y., Dyer, D., Seto, D., Rajaiya, J., and J. Chodosh. (2017). The 5' UTR in human adenoviruses: leader diversity in late gene expression. Scientific Reports 7(1):618.

Zhang, Q., Dehghan, S., and D. Seto. (2016). Pitfalls of restriction enzyme analysis in identifying, characterizing, typing, and naming viral pathogens in the era of whole genome data, as illustrated by HAdV type 55. Virologica Sinica 31: 448-453.

Zeng, A., Zhang, J., Jing, S., Cheng, Z., Girones, R., Seto, D.,* and Q. Zhang.* (2016). Genome Sequence of a Cynomolgus Macaque Adenovirus (CynAdV-1) Isolated From a Primate Colony in the United Kingdom. Genome Announcements 3:e01193-16. *corresponding authors.

Ismail, M.A., Lee, J.S., Dyer, D., Seto, D., Rajaiya, J. and J. Chodosh. (2016). Selection pressure in the human adenovirus fiber knob drives cell specificity in epidemic keratoconjunctivitis. J Virol. 90: 9598-9607.

Singh, G., Zhou, X., Lee, J.Y., Yousuf, M.A., Ramke, M., Ismail, M.A., Lee, J.S., Robinson, C.M., Seto, D., Dyer, D.W., Jones, M.S., Rajaiya, J. and J. Chodosh. (2015). Recombination of the epsilon determinant and corneal tropism: Human adenovirus species D types 15, 29, 56, and 69. Virology 485: 452–459.

Zhang, Q. and D. Seto. (2015). Chimpanzee adenovirus vector ebola vaccine- Preliminary Report. N. Engl. J. Med. 373: 775-6. (Commentary).

Adriaenssens, E.M., Edwards, R., Nash, J.H.E., Mahadevan, P., Seto, D., Ackermann, H.W., Lavigne, R. and A.M. Kropinski. (2015). Integration of genomic and proteomic analyses in the classification of the Siphoviridae family. Virology 477: 144-54.

Zhao, S., Wan, C., Ke, C., Seto, J., Dehghan, S., Zou, L., Zhou, J., Cheng, Z., Jing, S., Zeng, Z., Zhang, J., Wan, X., Wu, X., Zhao, W., Zhu, L., Seto, D.*, and Q. Zhang*. (2014). Re-emergent Human Adenovirus Genome Type 7d Caused an Acute Respiratory Disease Outbreak in Southern China After a Twenty-one Year Absence. Sci. Rep. 4:7365. *corresponding authors.

Adriaenssens, E.M., Edwards, R., Nash, J.H.E., Mahadevan, P., Seto, D., Ackermann, H.W., Lavigne, R., and A.M. Kropinski. (2014). Integration of genomic and proteomic analyses in the classification of the Siphoviridae family. Virology pii: S0042-6822(14)00471-1.

Seto., D., Jones, M.S., Dyer, D.W., J. Chodosh. (2013). Characterizing, typing and naming human adenovirus type 55 in the era of whole genome data. J. Clin. Virol. 58: 741-2. (Commentary).

Dehghan, S., Seto, J., Jones, M.S., Dyer, D.W., Chodosh, J. and D. Seto. (2013). Simian adenovirus type 35 has a recombinant genome comprising human and simian adenovirus sequences, which predicts its potential emergence as a human respiratory pathogen. Virol. 447: 265-273.

Singh, G., Robinson, C.M., Dehghan, S., Jones, M.S., Dyer, D.W., D. Seto. and Chodosh, J. (2013). Homologous recombination in E3 genes of human adenoviruses species D. J Virol. 87: 12481-8.

Dehghan, S., Seto, J., Liu, E.B., Walsh, M.P., Dyer, D.W., Chodosh, J. and D. Seto. (2013). Computational analysis of four human adenovirus type 4 genomes reveals molecular evolution through two interspecies recombination events. Virology 443: 197-207. (NOTE: Selected as a "Highlighted Article" by the Editors of Virology).

Robinson, C.M., Singh, G., Lee, J.Y., Dehghan, S., Rajaiya, J., Liu, E.B., Yousuf, M.A., Betensky, R.A., Dyer, D.W., Jones, M.S., Seto, D. and J. Chodosh. (2013). Molecular evolution of human adenoviruses. Scientific Reports 3:1812.

Tang, L., An, J., Xie, Z., Dehghan, S., Seto, D., Xu, W. and Y. Ji. (2013). Genome and bioinformatic analysis of a HAdV-B14p1 virus isolated from a baby with pneumonia in Beijing, China. PLoS One 8(3): e60345.

Robinson, C., Zhou, X., Rajaiya, J., Yousuf, M.A., Singh, G., DeSerres, J.A., Walsh, M.P., Wong, S., Seto, D., Dyer, D.W., Chodosh, J.* and M.S. Jones. (2013). Predicting the next pathogen: analysis of a novel human adenovirus. mBio 4(2): e00595-12. *corresponding author.

Turner, D., Reynolds, D., Seto, D.* and P. Mahadevan*. (2013). CoreGenes 3.5: A webserver for the determination of core genes from sets of viral and small bacterial genomes. BMC Research Notes 6:140. *corresponding authors.

Zhang, Q., Seto, D., Cao, B., Zhao, S., Zhao, W., Zhu, L. and C. Wan. (2012). Genome sequence of human adenovirus type 55, a re-emergent acute respiratory disease pathogen in China. J Virol. 86: 12441-2.

Qurei, L., Seto, D., Salah, Z. and M. Azzeh. (2012). A molecular epidemiology survey of respiratory adenoviruses circulating in children residing in Southern Palestine. PLoS One 7(8): e42732.

Zhang, Q., Seto, D., Zhao, S., Zhu, L., Zhao, W. and C. Wan. (2012). Genome Sequence of the First Human Adenovirus Type 14 Isolated in China. J Virol. 86: 7019-20.

Zhou, X., Robinson, C.M., Rajaiya, J., Deghan, S., Seto, D., Jones, M.S., Dyer, D.W. and J. Chodosh. (2012). Analysis of human adenovirus type 19 associated with epidemic keratoconjunctivitis and its reclassification as adenovirus type 64. Invest Ophthalmol Vis Sci. 53: 2804-2811.

Liu, E.B., Wadford, D.A., Seto, J., Vu, M., Hudson, N.R., Thrasher, L., Torres, S., Messenger, S.L., Dyer, D.W., Chodosh, J., Seto, D. and M.S. Jones. (2012). Computational and serologic analysis of novel and known viruses in species human adenovirus D in which serology and genomics do not correlate. PLoS One 7(3): e33212.

Zhang, Q., Zhang, X., Wu, H., Seto, D., Zhang, H-J, Chen, Z., Wan, C. and B-J. Zheng. (2012). Parental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BC. PLoS One 7(2): e31233.

Singh, G., Robinson, C.M., Dehghan, S., Schmidt, T., Seto, D., Jones, M.S., Dyer, D.W. and Chodosh, J. (2012). Over-reliance on the hexon gene leading to misclassification of human adenoviruses. J Virol. 86: 4693-4695. [Epub 02/01/12].

Dehghan, S., Liu, E.B., Seto, J., Torres, S., Hudson, N.R., Kajon, A., Metzgar, D.W., Dyer, D.W., Chodosh, J., Jones, M.S. and D. Seto. (2012). Five genome sequences of subspecies B1 human adenoviruses associated with acute respiratory disease. J Virol. 86: 635-636.

Dehghan, S., Seto, J., Hudson, N.R., Robinson, C.M., Jones, M.S., Dyer, D.W., Chodosh, J. and D. Seto. (2011). Complete genome sequence of human adenovirus prototype 17. J Virol. 85: 11540-11541. [Epub 10/07/11].

Liu, E.B., Ferreyra, L., Fischer, S.L., Pavan, J.V., Nates, S.V., Hudson, N.R., Tirado, D., Dyer, D.W., Chodosh, J., Seto, D. and M.S. Jones. (2011). Genomic and computational analysis of a novel human adenovirus with a serologically unique hexon and a recombinant fiber gene. PLoS One. 6(9):e24491

Akula, N., Baranova, A., Seto, D., Solka, J., Nalls, M.A., Singleton, A., Ferrucci, L., Tanaka, T., Bandinelli, S., Cho, Y.S., Kim, Y.J., Lee, J-Y., Han, B-G. and F.J. McMahon. (2011). A network-based approach to prioritize results from genome-wide association studies. PLoS One. 6(9):e24220

Walsh, M.P., Seto, J., Liu, E.B., Dehghan, S., Hudson, N.R., Lukashev, A.N., Ivanova, O., Chodosh, J., Dyer, D., Jones, M.S. and D. Seto. (2011). Computational analysis of two species C human adenoviruses provides evidence of a novel virus. J Clin Microbiol. 49: 3482-3490-993. [Epub 08/17/11].

Robinson, C.M., Seto, D., Jones, M.S., Dyer, D.W. and J. Chodosh. (2011). Molecular evolution of human species D adenoviruses. Infect, Genet Evol. 11:1208-17. [ePub 05/05/11]. (Invited review).

Seto, D., Chodosh, J., Brister, J.R., Jones, M.S. and members of the Adenovirus Research Community. (2011). Using the whole genome sequence to characterize and name human adenoviruses. J Virol. 85: 5701-2. [Epub 03/30/11]. (Editorial).

Robinson, C.M., Gurdeep, S., Henquell, C., Walsh, M.P., Peigue-Lafeuille, H., Seto, D., Jones, M.S., Dyer, D.W. and J. Chodosh. (2011). Computational analysis and identification of an emergent human adenovirus pathogen implicated in a respiratory fatality. Virology 409: 141-147. [Epub 11/05/10].

Mahadevan, P. and D. Seto. (2011). In silico bioinformatic tools for determining core genes from sets of genomes. Drug Development Research 72: 147-152. [Epub 12/16/10]. (Review).

Seto, D. (2010). Viral genomics and bioinformatics. Viruses 2: 2587-2593. (Editorial).

Mahadevan, P. and D. Seto. (2010). In silico bioinformatic tools for determining core genes from sets of genomes. Drug Development Research 72: 1-6. (Invited Review).

Brister, J.R., Bao, Y., Kuiken, C., Lefkowitz, E., Le Mercier, P., Leplae, R., Madupu, R., Scheuermann, R., Schobel, S., Seto, D., Shrivastava, S., Sterk, P., Zeng, Q., Klimke W. and T. Tatusova. (2010). Towards viral genome annotation standards, Report from the 2010 NCBI Annotation Workshop. Viruses 2(10): 2258-2268. (Editorial).

Mahadevan, P. and Seto, D. (2010). "Taxonomic parsing of bacteriophages using core genes and in silico proteome-based CGUG and applications to small bacterial genomes." Adv Exp Med Biol. 680: 379-385. in Advances in Experimental Medicine and Biology. Arabnia, H.R. (Ed.). ISBN 978-1-4419-5912-6. (Book chapter).

Torres, S., Chodosh, J., Seto, D. and M.S. Jones. (2010). The revolution in viral genomics as exemplified by the bioinformatic analysis of human adenoviruses. Viruses 2(7): 1367-1381.

Walsh, M.P., Seto, J., Tirado, D., Chodosh, J., Seto, D.* and Jones, M.S. (2010). Genomic analysis of human adenovirus type 18. Virology. in press 05/13/10. [Epub 06/09/10]. *corresponding author.

Seto, J*., Walsh, M.P*., Metzgar, D. and Seto, D. (2010). Computational analysis of adenovirus serotype 5 (HAdV-C5) from a HAdV coinfection shows genome stability after forty-five years of circulation. Virology. in press 05/13/10. [Epub 06/03/10]. *equal contributions.

Mahadevan, P. and Seto, D. (2010). Pairwise synteny analysis of bacterial genomes using web-based GeneOrder. BMC Research Notes 3:41. [Epub 02/23/10]

Arnold, J., Janoska, M., Kajon, A.E., Metzgar, D., Hudson, N.R., Torres, S., Harrach, B., Seto, D., Chodosh, J. and Jones, M.S. (2010). Genomic characterization of human adenovirus 36, a putative obesity agent. Virus Research 149: 152-161. [Epub 01/28/10].

Seto, J., Walsh, M.P., Mahadevan, P. , Zhang, Q. and D. Seto. (2010). Applying genomic and bionformatic resources to human adenovirus genomes for use in vaccine development and for applications in vector development for gene delivery. Viruses 2: 1-26.

Walsh, M.P., Seto, J., Jones, M.S., Chodosh, J., Xu, W.and D. Seto. (2010). Computational analysis identifies human adenovirus type 55 as a re-emergent acute respiratory disease pathogen. J Clin Microbiol. 48: 991-993. [Epub 12/30/09]

Mahadevan, P., Seto, J., Tibbetts, C. and D. Seto. (2010). Natural variants of human adenovirus type 3 provide evidence for relative genome stability across time and geographic space. Virology 397: 113-118. [Epub 11/23/09].

Lavigne, R., Darius, P., Summer, E.J., Seto, D., Madhadevan, P., Nilsson, A.S., Ackermann, H.W. and A.M. Kropinski. (2009). Classification of Myoviridae bacteriophages using protein sequence similarity. BMC Microbiology 9:224. [Epub 10/26/09]

Mahadevan, P., King, J.F. and D. Seto. (2009). CGUG: in silico proteome and genome parsing tool for the determination of "core" and unique genes in the analysis of genomes up to ca. 1.9 Mb. BMC Research Notes 2:168. [Epub 08/25/09]

Mahadevan, P., King, J.F. and Seto, D. (2009). Data mining pathogen genomes using GeneOrder, CoreGenes and CGUG: gene order, synteny, and in silico proteomes. International Journal of Computational Biology and Drug Design 2:100-114.

Robinson, C.M., Rajaiya, J., Walsh, M.P., Dyer, D.W., Seto, D., Jones, M.S. and Chodosh, J. (2009). Computational analysis of human adenovirus type 22 provides evidence for recombination between human adenovirus species D in the penton base gene. J Virology. Jun 24 [Epub ahead of print].

Mahadevan, P. and Seto, D. (2009). In silico proteome parsing analysis to identify core sets of proteins in bacteriophages to aid in their classification. Proceedings of the 2009 International Conference on Bioinformatics and Computational Biology. CSREA Press. H. R. Arabnia and H. Valafar, eds. (Las Vegas, NV. July 13-16, 2009).

Kropinski, A.M., Borodovsky, M., Carver, T.J., Cerdeno-Tarraga, A.M., Darling, A., Lomsadze, A., Mahadevan, P., Stothard, P., Seto, D., van Domselaar, G. and Wishart, D.S. (2009). "In silico Identification of Genes in Bacteriophage DNA." Methods Mol Biol. 502: 57-89.

Walsh, M.P., Chintakuntlawar, A., Robinson, C.M., Madisch, I., Harrach, B., Hudson, N.R., Torres, S., Schnurr, D., Heim, A., Chodosh, J., Seto, D. and M.S. Jones. (2009). Evidence of molecular evolution driven by recombination events influencing tropism and virulence in a novel human adenovirus that causes epidemic keratoconjunctivitis. PLoS ONE 4(6):e5635.

Seto, J., Walsh, M.P., Mahadevan, P., Purkayastha, A., Clark, J.M., Tibbetts, C. and D. Seto (2009). Genomic and bioinformatics analyses of HAdV-14p, a member of the human adenovirus B2 species. Virus Research 143: 94-105. [Epub 4/2/09].

Zhang, Q., Su, X., Seto, D., Zheng, B-J., Tian, X., Sheng, H., Li, H., Wang, Y. and R. Zhou. (2009). Construction and characterization of a replication-competent human adenovirus type 3-based vector for use as a live-vaccine candidate and a gene therapy vector. Vaccine 27: 1145-1153. [Epub 1/13/09].

Lavigne, R., Seto, D., Madhadevan, P., Ackermann, H.W. and A.M. Kropinski. (2008). Unifying classical and molecular taxonomy-based classification: A rational classification system for the Podoviridae using BLASTP-based tools. Research in Microbiology 159: 406-414. [Epub 4/18/08].

Mahadevan, P. and Seto, D. (2008). Data mining pathogen genomes using GeneOrder and CoreGenes: gene order, synteny, and proteomes. Proceedings of the 2008 International Conference on Bioinformatics and Computational Biology. CSREA Press. H. R. Arabnia and H. Valafar, eds. (Las Vegas, NV. July 14-17, 2008).

Mahadevan, P. and Seto, D. (2007). Comparative genomics using CoreGenes to analyze small bacterial genomes, with an application for the annotation of hypothetical proteins. Proceedings of the 2007 International Conference on Bioinformatics and Computational Biology. CSREA Press. H. R. Arabnia and H. Valafar, eds. (Las Vegas, NV. June 25-28, 2007).

Vora, G.J., Lin, B., Gratwick, K., Meador, C., Hansen, C., Tibbetts, C., Stenger, D.A., Irvine, M., Seto, D., Purkayastha, A., Freed, N., Gibson, M.L., Russell K.L. and Metzgar, D. (2006). Coinfections of multiple adenoviral species in previously vaccinated patients. Emerging Infectious Diseases 12: 921-930.

Lin, B., Wang, Z., Vora, G.J., Thornton, J.A., Schnur, J.M., Thach, D.C., Mueller, K.M., Ligler, A.G., Malanoski, A.P., Santiago, J., Walter, E.A., Agan, B.K., Metzgar, D., Seto, D., Daum, L.T., Kruzelock, R., Rowley, R.K., Hanson, E.H., Tibbetts, C. and Stenger, D.A. (2006). Broad spectrum respiratory tract pathogen identification using re-sequencing DNA microarrays. Genome Res. 16: 527-55.

Mahadevan, P. and Seto, D. (2006). Comparative genomics using web-based GeneOrder to determine synteny and to function as an annotation tool. Proceedings of the 2006 International Conference on Bioinformatics and Computational Biology. CSREA Press. H. R. Arabnia and H. Valafar, eds. 566-570. (Las Vegas, NV. June 26-29, 2006).

Purkayastha, A., Su, J., McGraw, J., Ditty, S.E., Carlisle, S., Hadfield, T.L., Seto, J., Russell, K.L., Tibbetts, C. and Seto, D. (2005). Genomic and bioinformatics analysis of HAdV-4vac and HAdV-7vac, two human adenovirus (HAdV) strains that constituted original prophylaxis against HAdV-related acute respiratory disease, a reemerging epidemic disease. J Clin Microbiol. 43: 3083-3094.

Purkayastha, A., Ditty, S.E., Su, J., McGraw, J., Hadfield, T.L., Tibbetts, C. and Seto, D. (2005). Genomic and bioinformatics analysis of HAdV-4, a human adenovirus that causes acute respiratory disease (ARD). Implications for gene therapy and vaccine vector development. J Virol. 79: 2559-2572.

Purkayastha, A., Su, J., Carlisle, S., Tibbetts, C. and Seto, D. (2005). Genomic and bioinformatics analysis of HAdV-7, a human adenovirus of species B1 that causes acute respiratory disease (ARD). Implications for human gene therapy. Virology 332:114-129.

Lauer, K.P., Llorente, I., Blair, E., Seto, J., Krasnov, V., Purkayastha, A., Ditty, S.E., Hadfield, T.L., Buck, C., Tibbetts, C. and Seto, D. (2004). Natural variation among human adenoviruses: Genome sequence and annotation of human adenovirus serotype 1 (HAdV-1). J Gen Virol. 85: 2615-2625.

Celamkoti, S., Kundeti, S., Purkayastha, A, Mazumder, R., Buck, C. and Seto, D. (2004). GeneOrder3.0: Software for comparing the order of genes in pairs of small bacterial genomes. BMC Bioinformatics 5:52.

Purkayastha, A., Celamkoti, S., Kundeti, S., Buck, C. and Seto, D. (2003). Comparative analyses of small bacterial genomes using global computational tools GeneOrder and CoreGenes. Proceedings of the Seventh Joint Conference on Information Sciences (Fifth Conference on Computational Biology and Genome Informatics). Research Triangle Park, NC. Sept 03. K. Chen, et. al., eds. 863-868.

Zafar, N., Mazumder, R. and Seto, D. (2002). Application of global computational tools GeneOrder and CoreGenes to comparative analyses of chordopoxvirus genomes. Proceedings of the Sixth Joint Conference on Information Sciences (Fourth Conference on Computational Biology and Genome Informatics). Durham, NC Mar 02. H. J. Caulfield, et. al., eds. 1186-1192. Also selected for reprinting @ Information Sciences 146 (1-4): 127-135.

Zafar, N., Mazumder, R. and Seto, D. (2002). CoreGenes: A computational tool for identifying and cataloging “core” genes in a set of small genomes. BMC Bioinformatics 3:12.

Zafar, N., Mazumder, R. and Seto, D. (2001). Comparisons of gene co-linearity in genomes using GeneOrder2.0. Trends in Biochemical Sciences 26: 514-516.

Mazumder, R., Kolaskar, A. and Seto, D. (2001). GeneOrder: Comparing the order of genes in small genomes. Bioinformatics 17: 162-166.

Book Chapters


Agan, B.K., Hanson, E.H., Kruzelock, R.P., Lin, B, Rowley, R.K., Seto, D., Stenger, D.A., Thornton, J., Tibbetts, C.J., Thach, D., Vora, G.J., Walter, E.A. and Wang, Z. (2005). Re-sequencing pathogen microarray. United States Patent Office application number 11/177,646. U.S. patent (07/02/05; approved; royalties paid 07-13).
Foreign Patent (Filed January 2007; approved).


Epidemics Outbreak Surveillance Consortium (as a contributing member- see complete author listing in acknowledgements):
Liu, J., Walter, E., Stenger, D., Thach, D., and Epidemic Outbreak Surveillance Consortium. (2006). Effects of two globin mRNA reduction methods on gene expression profiles from whole blood. J Molec Diagnos. 8: 551-558.

Thach, D.C., Agan, B.K., Olsen, C., Diao, J., Lin, B., Gomez, J., Jesse, M., Jenkins, Rowley, R.K., Hanson, E., Tibbetts, C., Stenger, D.A, Walter, E., and Epidemic Outbreak Surveillance Consortium. (2005). Surveillance of transcriptomes in basic military trainees with normal, febrile respiratory illness, and convalescent phenotypes. Genes Immun. 6: 588–595.

Davignon, L., Walter, E., Mueller, K.M., Barrozo, C.P., Stenger, D.A., Lin, B. and Epidemic Outbreak Surveillance Consortium. (2005). Use of resequencing oligonucleotide microarrays for identification of Streptococcus pyogenes and associated antibiotic resistance determinants. J Clin Microbiol. 43: 5690-5695.triphosphohydrolase from E. coli. J. Biol. Chem. 263: 1494-1499.


Kuo, C. L., Chen, M. L., Wang, K., Chou, C. K., Vernooij, B., Seto, D., Koop, B. F. and Hood, L. (1998). A conserved sequence block in murine and human T-cell receptor (TCR) J alpha region is a composite element that enhances TCR alpha enhancer activity and binds multiple nuclear factors. Proc. Natl. Acad. Sci., USA 95: 3839-3844.

Bunville, J., Krakowski, K. and Seto, D. (1997). Cerium oxide removes background fluorescent contaminants, "Green Smears," from glass plates used for automated fluorescence-based DNA sequencing. BioTechniques 22: 236-238.

Seto, D. and Weintraub, B. (1996). Rapid molecular diagnosis of mutations associated with generalized thyroid hormone resistance by PCR-coupled automated direct sequencing of genomic DNA: Detection of two novel mutations. Human Mutation 8: 247-257.

Boisclair, Y., Seto, D., Hsieh, S., Hurst, K. R. and Ooi. G. (1996). Organization and chromosomal localization of the gene encoding the acid-labile subunit of the insulin-like growth factor binding complex. Proc. Natl. Acad. Sci., USA 93: 10028-10033.

Ooi, G. T., Cohen, F. J., Hsieh, S., Seto, D., Rechler, M. M. and Boisclair, Y. (1995). Structure and regulation of the ALS gene. Progress in Growth Factor Research 6: 151-157.

Krakowski, K., Bunville, J., Seto, J., Baskin, D. and Seto, D. (1995). Rapid purification of fluorescent dye-labeled products in a 96-well format for high-throughput automated DNA sequencing. Nucl. Acids Res. 23: 4930-4931.

Seto, D., Seto, J., Deshpande, P. and Hood, L. (1995). Use of DMSO to resolve banding artifacts in fluorescent DNA sequencing. DNA Sequence 5: 131-140.

Seto, D., Koop, B. F., Deshpande, P., Howard, S., Seto, J., Wilk, E., Wang, K. and Hood, L. (1994). Organization, sequence, and function of 34.5 kilobases of genomic DNA encompassing several murine T-cell receptor a/d variable gene segments. Genomics 20: 258-266.

Koop, B. F., Rowen, L., Wang, K., Kuo, C.-L., Seto, D., Lenstra, J. A., Howard, S., Shan, W., Deshpande, P. and Hood, L. (1994). The human T-cell receptor TCRa/TCRd (Ca/Cd) region: organization, sequence and evolution of 97.6 kb of DNA. Genomics 19: 478-493.

Seto, D., Koop, B. F. and Hood, L. (1993). An experimentally-derived data set constructed for testing large-scale DNA sequence assembly algorithms. Genomics 15: 673-676.

Seto, D., Koop, B. F., Seto, J. and Hood, L. (1992). Complete nucleotide sequence of the cosmid vector pWE15A. Nucl. Acids Res. 20: 3786.

Koop, B. F., Wilson, R. K., Wang, K., Vernooij, B., Zaller, D., Kuo, C. L., Seto, D., Toda, M. and Hood, L. (1992). Organization, structure, and function of 95kb of DNA spanning the murine T-cell receptor Ca/Cd region. Genomics 13: 1209-1230.

Seto, D. and Rohrabacher, C. (1991). Development and application of a nonradioactive phosphorescent autoradiograph marker. BioTechniques 10: 562-572.

Seto, D. (1991). A temperature regulator for microtiter plates. Nucl. Acids Res. 19: 2506.

Seto, D. (1990). An improved method for sequencing double stranded plasmid DNA from minipreps using DMSO and modified template preparation. Nucl. Acids Res. 18: 5905-5906.

Seto, D., Rohrabacher, C., Seto, J. and Hood, L. (1990). Phosphorescent zinc sulfide is a nonradioactive alternative for marking autoradiograms. Analyt. Biochem. 189: 51-53.

Quirk, S., Seto, D., Bhatnagar, S. K., Gauss, P., Gold, L. and Bessman, M. J. (1989). Location and molecular cloning of the structural gene for the deoxyguanosine triphosphate triphosphohydrolase of E. coli. Molec. Microbiol. 3: 1391-1395.

Wiederrecht, G., Seto, D. and Parker, C. S. (1988). Molecular cloning, nucleotide sequencing, and characterization of the Saccharomyces cerevisiae heat-shock transcription factor. Cell 54: 841-853.

Seto, D., Bhatnagar, S. K. and Bessman, M. J. (1988). The purification and properties of deoxyguanosine triphosphate triphosphohydrolase from E. coli. J. Biol. Chem. 263: 1494-1499

Web-based Resources

General Interest

  • Web-based genome analysis tools
  • Adenovirus Genome Diversity Project
  • (
  • Poxvirus Genome Diversity Project
  • (
  • Human Adenovirus 1 data set as a "tar" download (upon request)
  • (
  • Cosmid 2-47 data set Genomics '93 as a "tar" download

  • MicroArray Data Excel Files Images 1 Images 2 Images 3 Images 4 Images 5

    Datasets for classes
    BINF739 unknown pathogen DNA sequences- Next Gen data <= (Illumina, 2018, both = ONE genome)

    BINF739 unknown pathogen DNA sequences (Sanger data, ca., 2003)


    Revised April 8, 2022

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